2UVH

Structure of a periplasmic oligogalacturonide binding protein from Yersinia enterocolitica in complex with saturated digalacturonic acid

2UVH の概要

• エントリーDOI: 10.2210/pdb2uvh/pdb
• 関連するPDBエントリー: 2UVG 2UVI 2UVJ
• 分子名称: ABC TYPE PERIPLASMIC SUGAR-BINDING PROTEIN, alpha-D-galactopyranuronic acid-(1-4)-alpha-D-galactopyranuronic acid (3 entities in total)
• 機能のキーワード: yersinia enterocolitica, periplasmic binding protein, togb, pectin degradation, digalacturonic acid, sugar-binding protein, sugar binding protein
• 由来する生物種: YERSINIA ENTEROCOLITICA
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 46705.84

構造登録者

Abbott, D.W.,Boraston, A.B. (登録日: 2007-03-10, 公開日: 2007-03-20, 最終更新日: 2024-05-08)

主引用文献

Abbott, D.W.,Boraston, A.B.
Specific Recognition of Saturated and 4,5-Unsaturated Hexuronate Sugars by a Periplasmic Binding Protein Involved in Pectin Catabolism.
J.Mol.Biol., 369:759-, 2007

PubMed Abstract: The process of pectin depolymerization by pectate lyases and glycoside hydrolases produced by pectinolytic organisms, particularly the phytopathogens from the genus Erwinia, is reasonably well understood. Indeed each extracellular and intracellular catabolic stage has been identified using either genetic, bioinformatic or biochemical approaches. Nevertheless, the molecular details of many of these stages remain unknown. In particular, the mechanism and ligand binding profiles for the transport of pectin degradation products between cellular compartments remain entirely uninvestigated. Here we present the structure of TogB, a 45.7 kDa periplasmic binding protein from Yersinia enterocolitica. This protein is a component of the TogMNAB ABC transporter involved in the periplasmic transport of oligogalacturonides. In addition to the unliganded complex (at 2.2 A), we have also determined the structures of TogB in complex with digalacturonic acid (at 2.2 A), trigalacturonic acid (at 1.8 A) and 4,5-unsaturated digalacutronic acid (at 2.3 A). The molecular determinants of oligogalacturonide binding include a novel salt-bridge between the non-reducing sugar uronate group, selectivity for the unsaturated ligand, and the overall sugar configuration. Complementing this are UV difference and isothermal titration calorimetry experiments that highlight the thermodynamic basis of ligand specificity. The ligand binding profiles of the TogMNAB transporter complex nicely complement pectate lyase-mediated pectin degradation, which is a significant component of pectin depolymerization reactions.

PubMed: 17451747
DOI: 10.1016/J.JMB.2007.03.045

実験手法

X-RAY DIFFRACTION (2.2 Å)