2TOB

SOLUTION STRUCTURE OF THE TOBRAMYCIN-RNA APTAMER COMPLEX, NMR, 13 STRUCTURES

2TOB の概要

• エントリーDOI: 10.2210/pdb2tob/pdb
• 分子名称: RNA (5'-R(*AP*CP*UP*UP*GP*GP*UP*UP*UP*AP*GP*GP*UP*AP*AP*UP*GP*AP*GP*U)-3'), 3-ammonio-3-deoxy-alpha-D-glucopyranose, 2,6-diammonio-2,3,6-trideoxy-alpha-D-glucopyranose, ... (4 entities in total)
• 機能のキーワード: aminoglycoside-rna recognition, tobramycin, rna
• 由来する生物種: synthetic construct
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6935.40

構造登録者

Jiang, L.,Patel, D.J. (登録日: 1998-06-17, 公開日: 1999-06-22, 最終更新日: 2024-05-01)

主引用文献

Jiang, L.,Patel, D.J.
Solution structure of the tobramycin-RNA aptamer complex.
Nat.Struct.Biol., 5:769-774, 1998

PubMed Abstract: We have solved the solution structure of the aminoglycoside antibiotic tobramycin complexed with a stem-loop RNA aptamer. The 14 base loop of the RNA aptamer 'zippers up' alongside the attached stem through alignment of four mismatches and one Watson-Crick pair on complex formation. The tobramycin inserts into the deep groove centered about the mismatch pairs and is partially encapsulated between its floor and a looped out guanine base that flaps over the bound antibiotic. Several potential intermolecular hydrogen bonds between the charged NH3 groups of tobramycin and acceptor atoms on base pair edges and backbone phosphates anchor the aminoglycoside antibiotic within its sequence/structure specific RNA binding pocket.

PubMed: 9731769
DOI: 10.1038/1804

実験手法

SOLUTION NMR