2TGF

THE SOLUTION STRUCTURE OF HUMAN TRANSFORMING GROWTH FACTOR ALPHA

2TGF の概要

• エントリーDOI: 10.2210/pdb2tgf/pdb
• 分子名称: TRANSFORMING GROWTH FACTOR-ALPHA (1 entity in total)
• 機能のキーワード: growth factor
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Transforming growth factor alpha: Secreted, extracellular space. Protransforming growth factor alpha: Cell membrane; Single-pass type I membrane protein: P01135
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5560.25

構造登録者

Harvey, T.S.,Wilkinson, A.J.,Tappin, M.J.,Cooke, R.M.,Campbell, I.D. (登録日: 1991-01-23, 公開日: 1993-04-15, 最終更新日: 2024-10-23)

主引用文献

Harvey, T.S.,Wilkinson, A.J.,Tappin, M.J.,Cooke, R.M.,Campbell, I.D.
The solution structure of human transforming growth factor alpha.
Eur.J.Biochem., 198:555-562, 1991

PubMed Abstract: The solution structure of transforming growth factor alpha has been determined by a combination of high-resolution 1H-nuclear magnetic resonance and distance geometry and restrained molecular dynamics. The 382 restraints derived from the NMR experiments were used to calculate many distance geometry structures, which were then refined by restrained molecular mechanics. Five of these structures were further refined using a variety of methods. Comparison of independently measured parameters, such as calculated hydrogen bonding patterns and experimental amide exchange rates, have been used to evaluate the accuracy of the structures. Also, possible mechanisms to explain the pH-dependent conformational interconversion observed are suggested. Finally comparisons between this work and others on this topic have been made.

PubMed: 2050136
DOI: 10.1111/j.1432-1033.1991.tb16050.x

実験手法

SOLUTION NMR