2SFA

SERINE PROTEINASE FROM STREPTOMYCES FRADIAE ATCC 14544

2SFA の概要

• エントリーDOI: 10.2210/pdb2sfa/pdb
• 分子名称: SERINE PROTEINASE (2 entities in total)
• 機能のキーワード: hydrolase, serine protease
• 由来する生物種: Streptomyces fradiae
• 細胞内の位置: Secreted: P41140
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 19014.66

構造登録者

Kitadokoro, K.,Tsuzuki, H. (登録日: 1994-04-25, 公開日: 1996-06-20, 最終更新日: 2024-11-20)

主引用文献

Kitadokoro, K.,Tsuzuki, H.,Nakamura, E.,Sato, T.,Teraoka, H.
Purification, characterization, primary structure, crystallization and preliminary crystallographic study of a serine proteinase from Streptomyces fradiae ATCC 14544.
Eur.J.Biochem., 220:55-61, 1994

PubMed Abstract: A proteinase having wide substrate specificity was isolated from Streptomyces fradiae ATCC 14544. This proteinase, which we propose to call SFase-2, was purified from the culture filtrate by S-Sepharose chromatography. The purified enzyme showed an apparent molecular mass of 19 kDa on SDS/PAGE. When synthetic peptides were used as substrates, SFase-2 showed broad substrate specificity. It also hydrolyzed keratin, elastin and collagen as proteinaceous substrates. It was completely inhibited by diisopropylfluorophosphate and chymostatin, but not by tosylphenylalaninechloromethane, tosyllysinechloromethane or EDTA, indicating that it can be classified as a serine proteinase. The matured protein sequence of SFase-2 was determined by a combination of amino acid sequencing and the DNA sequencing of the gene. SFase-2, consisting of 191 amino acids, is a novel proteinase. It showed 76% similarity in the amino acid sequence with Streptomyces griseus proteinase A [Johnson P. and Smillie L. B. (1974) FEBS Lett. 47, 1-6]. For insight into the three-dimensional structure of SFase-2, we obtained single crystals by the vapor diffusion method using sodium phosphate as a precipitant. These crystals belonged to the orthorhombic, space group P2(1)2(1)2(1) with cell dimensions a = 6.92 nm, b = 7.28 nm, c = 2.99 nm; one molecule was present in the asymmetric unit.

PubMed: 8119298
DOI: 10.1111/j.1432-1033.1994.tb18598.x

実験手法

X-RAY DIFFRACTION (1.6 Å)