2RV8

Solution Structure of the PhoP DNA-Binding Domain from Mycobacterium tuberculosis

2RV8 の概要

• エントリーDOI: 10.2210/pdb2rv8/pdb
• NMR情報: BMRB: 11590
• 分子名称: DNA-binding response regulator (1 entity in total)
• 機能のキーワード: phopc, heteronuclear noe, mtb, dna binding protein
• 由来する生物種: Mycobacterium tuberculosis CAS/NITR204
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14723.73

構造登録者

Macdonald, R.,Sarkar, D.,Amer, B.R.,Clubb, R.T. (登録日: 2015-04-17, 公開日: 2015-08-12, 最終更新日: 2024-05-15)

主引用文献

Macdonald, R.,Sarkar, D.,Amer, B.R.,Clubb, R.T.
Solution structure of the PhoP DNA-binding domain from Mycobacterium tuberculosis.
J.Biomol.Nmr, 63:111-117, 2015

PubMed Abstract: Tuberculosis caused by is a leading cause of death world-wide. The PhoP protein is required for virulence and is part of the PhoPR two-component system that regulates gene expression. The NMR-derived solution structure of the PhoP C-terminal DNA-binding domain is reported. Residues 150 to 246 form a structured domain that contains a winged helix-turn-helix motif. We provide evidence that the transactivation loop postulated to contact RNA polymerase is partially disordered in solution, and that the polypeptide that connects the DNA-binding domain to the regulatory domain is unstructured.

PubMed: 26209027
DOI: 10.1007/s10858-015-9965-0

実験手法

SOLUTION NMR