2RUP

Solution structure of rat P2X4 receptor head domain

Summary for 2RUP

• Entry DOI: 10.2210/pdb2rup/pdb
• NMR Information: BMRB: 11583
• Descriptor: P2X purinoceptor 4 (1 entity in total)
• Functional Keywords: p2x4 receptor, head domain, canion transport, metal ion binding, transport protein
• Biological source: Rattus norvegicus (brown rat,rat,rats)
• Total number of polymer chains: 1
• Total formula weight: 6066.72

Authors

Abe, Y.,Igawa, T.,Tsuda, M.,Inoue, K.,Ueda, T. (deposition date: 2014-11-12, release date: 2015-02-04, Last modification date: 2024-10-16)

Primary citation

Igawa, T.,Abe, Y.,Tsuda, M.,Inoue, K.,Ueda, T.
Solution structure of the rat P2X4 receptor head domain involved in inhibitory metal binding
FEBS Lett., 589:680-686, 2015

PubMed Abstract: The P2X receptor is an ATP-gated cation channel expressed on the plasma membrane. The head domain (Gln111-Val167 in the rat P2X4 receptor) regulates ATP-induced cation influx. In this study, we prepared a head domain with three disulfide bonds, such as the intact rat P2X4 receptor contains. NMR analysis showed that the head domain possessed the same fold as in the zebrafish P2X4 receptor previously determined by crystallography. Furthermore, the inhibitory, divalent, metal ion binding sites were determined by NMR techniques. These findings will be useful for the design of specific inhibitors for the P2X receptor family.

PubMed: 25662851
DOI: 10.1016/j.febslet.2015.01.034

Experimental method

SOLUTION NMR