2RUP

Solution structure of rat P2X4 receptor head domain

2RUP の概要

• エントリーDOI: 10.2210/pdb2rup/pdb
• NMR情報: BMRB: 11583
• 分子名称: P2X purinoceptor 4 (1 entity in total)
• 機能のキーワード: p2x4 receptor, head domain, canion transport, metal ion binding, transport protein
• 由来する生物種: Rattus norvegicus (brown rat,rat,rats)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6066.72

構造登録者

Abe, Y.,Igawa, T.,Tsuda, M.,Inoue, K.,Ueda, T. (登録日: 2014-11-12, 公開日: 2015-02-04, 最終更新日: 2024-10-16)

主引用文献

Igawa, T.,Abe, Y.,Tsuda, M.,Inoue, K.,Ueda, T.
Solution structure of the rat P2X4 receptor head domain involved in inhibitory metal binding
FEBS Lett., 589:680-686, 2015

PubMed Abstract: The P2X receptor is an ATP-gated cation channel expressed on the plasma membrane. The head domain (Gln111-Val167 in the rat P2X4 receptor) regulates ATP-induced cation influx. In this study, we prepared a head domain with three disulfide bonds, such as the intact rat P2X4 receptor contains. NMR analysis showed that the head domain possessed the same fold as in the zebrafish P2X4 receptor previously determined by crystallography. Furthermore, the inhibitory, divalent, metal ion binding sites were determined by NMR techniques. These findings will be useful for the design of specific inhibitors for the P2X receptor family.

PubMed: 25662851
DOI: 10.1016/j.febslet.2015.01.034

実験手法

SOLUTION NMR