2RUG

Refined solution structure of the first RNA recognition motif domain in CPEB3

Summary for 2RUG

• Entry DOI: 10.2210/pdb2rug/pdb
• NMR Information: BMRB: 11563
• Descriptor: Cytoplasmic polyadenylation element-binding protein 3 (1 entity in total)
• Functional Keywords: rrm domain, rbd, rna binding protein, structural genomics, riken structural genomics/proteomics initiative, rsgi
• Biological source: Homo sapiens (human)
• Total number of polymer chains: 1
• Total formula weight: 12428.83

Authors

Tsuda, K.,Kuwasako, K.,Nagata, T.,Takahashi, M.,Kigawa, T.,Kobayashi, N.,Guntert, P.,Shirouzu, M.,Yokoyama, S.,Muto, Y.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2014-04-15, release date: 2014-09-17, Last modification date: 2024-05-15)

Primary citation

Tsuda, K.,Kuwasako, K.,Nagata, T.,Takahashi, M.,Kigawa, T.,Kobayashi, N.,Guntert, P.,Shirouzu, M.,Yokoyama, S.,Muto, Y.
Novel RNA recognition motif domain in the cytoplasmic polyadenylation element binding protein 3.
Proteins, 82:2879-2886, 2014

PubMed Abstract: The family of cytoplasmic polyadenylation element binding proteins CPEB1, CPEB2, CPEB3, and CPEB4 binds to the 3'-untranslated region (3'-UTR) of mRNA, and plays significant roles in mRNA metabolism and translation regulation. They have a common domain organization, involving two consecutive RNA recognition motif (RRM) domains followed by a zinc finger domain in the C-terminal region. We solved the solution structure of the first RRM domain (RRM1) of human CPEB3, which revealed that CPEB3 RRM1 exhibits structural features distinct from those of the canonical RRM domain. Our structural data provide important information about the RNA binding ability of CPEB3 RRM1.

PubMed: 25066254
DOI: 10.1002/prot.24651

Experimental method

SOLUTION NMR