2RU9

Structure of the YAM domain of E. coli Transporter YajR

2RU9 の概要

• エントリーDOI: 10.2210/pdb2ru9/pdb
• NMR情報: BMRB: 11555
• 分子名称: Predicted transporter (1 entity in total)
• 機能のキーワード: ferredoxin like, transport protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8704.83

構造登録者

Liu, X.,Feng, W. (登録日: 2014-02-27, 公開日: 2014-07-02, 最終更新日: 2024-05-15)

主引用文献

Jiang, D.,Zhao, Y.,Fan, J.,Liu, X.,Wu, Y.,Feng, W.,Zhang, X.C.
Atomic resolution structure of the E. coli YajR transporter YAM domain.
Biochem.Biophys.Res.Commun., 450:929-935, 2014

PubMed Abstract: YajR is an Escherichia coli transporter that belongs to the major facilitator superfamily. Unlike most MFS transporters, YajR contains a carboxyl terminal, cytosolic domain of 67 amino acid residues termed YAM domain. Although it is speculated that the function of this small soluble domain is to regulate the conformational change of the 12-helix transmembrane domain, its precise regulatory role remains unclear. Here, we report the crystal structure of the YAM domain at 1.07-Å resolution, along with its structure determined using nuclear magnetic resonance. Detailed analysis of the high resolution structure revealed a symmetrical dimer in which a belt of well-ordered poly-pentagonal water molecules is embedded. A mutagenesis experiment and a thermal stability assay were used to analyze the putative role of this dimerization in response to changes in halogen concentration.

PubMed: 24952155
DOI: 10.1016/j.bbrc.2014.06.053

実験手法

SOLUTION NMR