2RU6

The pure alternative state of ubiquitin

2RU6 の概要

• エントリーDOI: 10.2210/pdb2ru6/pdb
• 関連するPDBエントリー: 2RSU
• NMR情報: BMRB: 11547
• 分子名称: Ubiquitin (1 entity in total)
• 機能のキーワード: ubiquitin, alternatively folded state, high-pressure nmr, q41n variant, protein binding
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Ubiquitin: Cytoplasm (By similarity): P0CG48
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 8562.81

構造登録者

Kitazawa, S.,Kameda, T.,Kumo, A.,Utsumi, M.,Baxter, N.,Kato, K.,Williamson, M.P.,Kitahara, R. (登録日: 2013-12-04, 公開日: 2014-02-12, 最終更新日: 2024-05-15)

主引用文献

Kitazawa, S.,Kameda, T.,Kumo, A.,Yagi-Utsumi, M.,Baxter, N.J.,Kato, K.,Williamson, M.P.,Kitahara, R.
Close Identity between Alternatively Folded State N2 of Ubiquitin and the Conformation of the Protein Bound to the Ubiquitin-Activating Enzyme
Biochemistry, 53:447-449, 2014

PubMed Abstract: We present the nuclear Overhauser effect-based structure determination of the Q41N variant of ubiquitin at 2500 bar, where the alternatively folded N2 state is 97% populated. This allows us to characterize the structure of the "pure" N2 state of ubiquitin. The N2 state shows a substantial change in the orientation of strand β5 compared to that of the normal folded N1 state, which matches the changes seen upon binding of ubiquitin to ubiquitin-activating enzyme E1. The recognition of E1 by ubiquitin is therefore best explained by conformational selection rather than induced-fit motion.

PubMed: 24401037
DOI: 10.1021/bi401617n

実験手法

SOLUTION NMR