2RT6

Backbone 1H, 13C, and 15N Chemical Shift Assignments for PriC N-terminal domain

2RT6 の概要

• エントリーDOI: 10.2210/pdb2rt6/pdb
• NMR情報: BMRB: 11525
• 分子名称: Primosomal replication protein N'' (1 entity in total)
• 機能のキーワード: primosome, replication restart, pric, dna binding protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 11007.53

構造登録者

Aramaki, T.,Abe, Y.,Katayama, T.,Ueda, T. (登録日: 2013-04-24, 公開日: 2013-08-07, 最終更新日: 2024-05-15)

主引用文献

Aramaki, T.,Abe, Y.,Katayama, T.,Ueda, T.
Solution structure of the N-terminal domain of a replication restart primosome factor, PriC, in Escherichia coli.
Protein Sci., 22:1279-1286, 2013

PubMed Abstract: In eubacterial organisms, the oriC-independent primosome plays an essential role in replication restart after the dissociation of the replication DNA-protein complex by DNA damage. PriC is a key protein component in the replication restart primosome. Our recent study suggested that PriC is divided into two domains: an N-terminal and a C-terminal domain. In the present study, we determined the solution structure of the N-terminal domain, whose structure and function have remained unknown until now. The revealed structure was composed of three helices and one extended loop. We also observed chemical shift changes in the heteronuclear NMR spectrum and oligomerization in the presence of ssDNA. These abilities may contribute to the PriC-ssDNA complex, which is important for the replication restart primosome.

PubMed: 23868391
DOI: 10.1002/pro.2314

実験手法

SOLUTION NMR