2RT3

Solution structure of the second RRM domain of Nrd1

2RT3 の概要

• エントリーDOI: 10.2210/pdb2rt3/pdb
• NMR情報: BMRB: 11523
• 分子名称: Negative regulator of differentiation 1 (1 entity in total)
• 機能のキーワード: nrd1, rna-binding, rna binding protein
• 由来する生物種: Schizosaccharomyces pombe (Fission yeast)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 10793.34

構造登録者

Kobayashi, A.,Kanaba, T.,Mishima, M. (登録日: 2013-04-16, 公開日: 2014-04-16, 最終更新日: 2024-05-01)

主引用文献

Kobayashi, A.,Kanaba, T.,Satoh, R.,Fujiwara, T.,Ito, Y.,Sugiura, R.,Mishima, M.
Structure of the second RRM domain of Nrd1, a fission yeast MAPK target RNA binding protein, and implication for its RNA recognition and regulation
Biochem.Biophys.Res.Commun., 437:12-17, 2013

PubMed Abstract: Negative regulator of differentiation 1 (Nrd1) is known as a negative regulator of sexual differentiation in fission yeast. Recently, it has been revealed that Nrd1 also regulates cytokinesis, in which physical separation of the cell is achieved by a contractile ring comprising many proteins including actin and myosin. Cdc4, a myosin II light chain, is known to be required for cytokinesis. Nrd1 binds and stabilizes Cdc4 mRNA, and thereby suppressing the cytokinesis defects of the cdc4 mutants. Interestingly, Pmk1 MAPK phosphorylates Nrd1, resulting in markedly reduced RNA binding activity. Furthermore, Nrd1 localizes to stress granules in response to various stresses, and Pmk1 phosphorylation enhances the localization. Nrd1 consists of four RRM domains, although the mechanism by which Pmk1 regulates the RNA binding activity of Nrd1 is unknown. In an effort to delineate the relationship between Nrd1 structure and function, we prepared each RNA binding domain of Nrd1 and examined RNA binding to chemically synthesized oligo RNA using NMR. The structure of the second RRM domain of Nrd1 was determined and the RNA binding site on the second RRM domain was mapped by NMR. A plausible mechanism pertaining to the regulation of RNA binding activity by phosphorylation is also discussed.

PubMed: 23770370
DOI: 10.1016/j.bbrc.2013.06.008

実験手法

SOLUTION NMR