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2RSG

Solution structure of the CERT PH domain

Summary for 2RSG
Entry DOI10.2210/pdb2rsg/pdb
NMR InformationBMRB: 11473
DescriptorCollagen type IV alpha-3-binding protein (1 entity in total)
Functional Keywordspleckstrin homology, lipid transport
Biological sourceHomo sapiens (human)
Cellular locationCytoplasm: Q9Y5P4
Total number of polymer chains1
Total formula weight11180.38
Authors
Sugiki, T.,Takeuchi, K.,Tokunaga, Y.,Kumagai, K.,Kawano, M.,Nishijima, M.,Hanada, K.,Takahashi, H.,Shimada, I. (deposition date: 2012-02-25, release date: 2012-08-15, Last modification date: 2024-05-15)
Primary citationSugiki, T.,Takeuchi, K.,Yamaji, T.,Takano, T.,Tokunaga, Y.,Kumagai, K.,Hanada, K.,Takahashi, H.,Shimada, I.
Structural basis for the Golgi association by the pleckstrin homology domain of the ceramide trafficking protein (CERT)
J.Biol.Chem., 287:33706-33718, 2012
Cited by
PubMed Abstract: Ceramide transport from the endoplasmic reticulum to the Golgi apparatus is crucial in sphingolipid biosynthesis, and the process relies on the ceramide trafficking protein (CERT), which contains pleckstrin homology (PH) and StAR-related lipid transfer domains. The CERT PH domain specifically recognizes phosphatidylinositol 4-monophosphate (PtdIns(4)P), a characteristic phosphoinositide in the Golgi membrane, and is indispensable for the endoplasmic reticulum-to-Golgi transport of ceramide by CERT. In this study, we determined the three-dimensional structure of the CERT PH domain by using solution NMR techniques. The structure revealed the presence of a characteristic basic groove near the canonical PtdIns(4)P recognition site. An extensive interaction study using NMR and other biophysical techniques revealed that the basic groove coordinates the CERT PH domain for efficient PtdIns(4)P recognition and localization in the Golgi apparatus. The notion was also supported by Golgi mislocalization of the CERT mutants in living cells. The distinctive binding modes reflect the functions of PH domains, as the basic groove is conserved only in the PH domains involved with the PtdIns(4)P-dependent lipid transport activity but not in those with the signal transduction activity.
PubMed: 22869376
DOI: 10.1074/jbc.M112.367730
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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数据于2025-06-25公开中

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