2RS7

Solution structure of the second dsRBD from RNA helicase A

2RS7 の概要

• エントリーDOI: 10.2210/pdb2rs7/pdb
• 関連するPDBエントリー: 1UIL 2RS6
• NMR情報: BMRB: 11457
• 分子名称: ATP-dependent RNA helicase A (1 entity in total)
• 機能のキーワード: double-stranded rna binding domain, dsrbd, dsrm, hydrolase, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Nucleus, nucleolus: O70133
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12506.93

構造登録者

Nagata, T.,Muto, Y.,Tsuda, K.,Inoue, M.,Kigawa, T.,Terada, T.,Shirouzu, M.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2011-11-29, 公開日: 2012-03-14, 最終更新日: 2024-05-15)

主引用文献

Nagata, T.,Tsuda, K.,Kobayashi, N.,Shirouzu, M.,Kigawa, T.,Guntert, P.,Yokoyama, S.,Muto, Y.
Solution structures of the double-stranded RNA-binding domains from RNA helicase A
Proteins, 80:1699-1706, 2012

PubMed Abstract: RNA helicase A (RHA) is a highly conserved protein with multifaceted functions in the gene expression of cellular and viral mRNAs. RHA recognizes highly structured nucleotides and catalytically rearranges the various interactions between RNA, DNA, and protein molecules to provide a platform for the ribonucleoprotein complex. We present the first solution structures of the double-stranded RNA-binding domains (dsRBDs), dsRBD1 and dsRBD2, from mouse RHA. We discuss the binding mode of the dsRBDs of RHA, in comparison with the known dsRBD structures in their complexes. Our structural data provide important information for the elucidation of the molecular reassembly mediated by RHA.

PubMed: 22454253
DOI: 10.1002/prot.24059

実験手法

SOLUTION NMR