2RR4

Complex structure of the zf-CW domain and the H3K4me3 peptide

2RR4 の概要

• エントリーDOI: 10.2210/pdb2rr4/pdb
• 関連するPDBエントリー: 2E61
• NMR情報: BMRB: 11115
• 分子名称: Zinc finger CW-type PWWP domain protein 1, Histone H3, ZINC ION (3 entities in total)
• 機能のキーワード: zf-cw domain, zcwpw1, zinc-finger, structural genomics, nppsfa, national project on protein structural and functional analyses, riken structural genomics/proteomics initiative, rsgi, metal-binding protein-nuclear protein complex, metal-binding protein/nuclear protein
• 由来する生物種: Homo sapiens (human); 詳細
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 8906.17

構造登録者

He, F.,Muto, Y.,Inoue, M.,Kigawa, T.,Shirouzu, M.,Terada, T.,Yokoyama, S.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (登録日: 2010-03-24, 公開日: 2010-09-15, 最終更新日: 2025-03-26)

主引用文献

He, F.,Umehara, T.,Saito, K.,Harada, T.,Watanabe, S.,Yabuki, T.,Kigawa, T.,Takahashi, M.,Kuwasako, K.,Tsuda, K.,Matsuda, T.,Aoki, M.,Seki, E.,Kobayashi, N.,Guntert, P.,Yokoyama, S.,Muto, Y.
Structural insight into the zinc finger CW domain as a histone modification reader
Structure, 18:1127-1139, 2010

PubMed Abstract: The zinc finger CW (zf-CW) domain is a motif of about 60 residues that is frequently found in proteins involved in epigenetic regulation. Here, we determined the NMR solution structure of the zf-CW domain of the human zf-CW and PWWP domain containing protein 1 (ZCWPW1). The zf-CW domain adopts a new fold in which a zinc ion is coordinated tetrahedrally by four conserved Cys ligand residues. The tertiary structure of the zf-CW domain partially resembles that adopted by the plant homeo domain (PHD) finger bound to the histone tail, suggesting that the zf-CW domain and the PHD finger have similar functions. The solution structure of the complex of the zf-CW domain with the histone H3 tail peptide (1-10) with trimethylated K4 clarified its binding mode. Our structural and biochemical studies have identified the zf-CW domain as a member of the histone modification reader modules for epigenetic regulation.

PubMed: 20826339
DOI: 10.1016/j.str.2010.06.012

実験手法

SOLUTION NMR