2RQQ

Structure of C-terminal region of Cdt1

2RQQ の概要

• エントリーDOI: 10.2210/pdb2rqq/pdb
• 分子名称: DNA replication factor Cdt1 (1 entity in total)
• 機能のキーワード: licensing factor, cell cycle, dna replication, dna-binding, proto-oncogene
• 由来する生物種: Mus musculus (mouse)
• 細胞内の位置: Nucleus: Q8R4E9
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12751.95

構造登録者

Jee, J.G.,Mizuno, T.,Kamada, K.,Tochio, H.,Hiroaki, H.,Hanaoka, F.,Shirakawa, M. (登録日: 2009-10-14, 公開日: 2010-03-23, 最終更新日: 2024-05-29)

主引用文献

Jee, J.G.,Mizuno, T.,Kamada, K.,Tochio, H.,Chiba, Y.,Yanagi, K.,Yasuda, G.,Hiroaki, H.,Hanaoka, F.,Shirakawa, M.
Structure and mutagenesis studies of the C-terminal region of licensing factor Cdt1 enable the identification of key residues for binding to replicative helicase Mcm proteins
J.Biol.Chem., 285:15931-15940, 2010

PubMed Abstract: In eukaryotes, DNA replication is fired once in a single cell cycle before cell division starts to maintain stability of the genome. This event is tightly controlled by a series of proteins. Cdt1 is one of the licensing factors and is involved in recruiting replicative DNA helicase Mcm2-7 proteins into the pre-replicative complex together with Cdc6. In Cdt1, the C-terminal region serves as a binding site for Mcm2-7 proteins, although the details of these interactions remain largely unknown. Here, we report the structure of the region and the key residues for binding to Mcm proteins. We determined the solution structure of the C-terminal fragment, residues 450-557, of mouse Cdt1 by NMR. The structure consists of a winged-helix domain and shows unexpected similarity to those of the C-terminal domain of Cdc6 and the central fragment of Cdt1, thereby implying functional and evolutionary relationships. Structure-based mutagenesis and an in vitro binding assay enabled us to pinpoint the region that interacts with Mcm proteins. Moreover, by performing in vitro binding and budding yeast viability experiments, we showed that approximately 45 residues located in the N-terminal direction of the structural region are equally crucial for recognizing Mcm proteins. Our data suggest the possibility that winged-helix domain plays a role as a common module to interact with replicative helicase in the DNA replication-licensing process.

PubMed: 20335175
DOI: 10.1074/jbc.M109.075333

実験手法

SOLUTION NMR