2RPI

The NMR structure of the submillisecond folding intermediate of the Thermus thermophilus ribonuclease H

2RPI の概要

• エントリーDOI: 10.2210/pdb2rpi/pdb
• 関連するPDBエントリー: 1QF8
• 分子名称: Ribonuclease H (1 entity in total)
• 機能のキーワード: submillisecond folding intermediate, thermus thermophilus ribonuclease h, high-resolution structure, endonuclease, hydrolase, magnesium, metal-binding, nuclease
• 由来する生物種: Thermus thermophilus HB8
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 12878.81

構造登録者

Zhou, Z.,Feng, H.,Bai, Y. (登録日: 2008-05-16, 公開日: 2009-03-31, 最終更新日: 2024-05-29)

主引用文献

Zhou, Z.,Feng, H.,Ghirlando, R.,Bai, Y.
The high-resolution NMR structure of the early folding intermediate of the Thermus thermophilus ribonuclease H
J.Mol.Biol., 384:531-539, 2008

PubMed Abstract: Elucidation of the high-resolution structures of folding intermediates is a necessary but difficult step toward the ultimate understanding of the mechanism of protein folding. Here, using hydrogen-exchange-directed protein engineering, we populated the folding intermediate of the Thermus thermophilus ribonuclease H, which forms before the rate-limiting transition state, by removing the unfolded regions of the intermediate, including an alpha-helix and two beta-strands (51 folded residues). Using multidimensional NMR, we solved the structure of this intermediate mimic to an atomic resolution (backbone rmsd, 0.51 A). It has a native-like backbone topology and shows some local deviations from the native structure, revealing that the structure of the folded region of an early folding intermediate can be as well defined as the native structure. The topological parameters calculated from the structures of the intermediate mimic and the native state predict that the intermediate should fold on a millisecond time scale or less and form much faster than the native state. Other factors that may lead to the slow folding of the native state and the accumulation of the intermediate before the rate-limiting transition state are also discussed.

PubMed: 18848567
DOI: 10.1016/j.jmb.2008.09.044

実験手法

SOLUTION NMR