2RO1

NMR Solution Structures of Human KAP1 PHD finger-bromodomain

2RO1 の概要

• エントリーDOI: 10.2210/pdb2ro1/pdb
• 分子名称: Transcription intermediary factor 1-beta, ZINC ION (2 entities in total)
• 機能のキーワード: kap, tif, phd finger, bromodomain, sumo, acetylation, alternative splicing, metal-binding, nucleus, phosphoprotein, repressor, transcription, transcription regulation, zinc, zinc-finger
• 由来する生物種: Homo sapiens (Human)
• 細胞内の位置: Nucleus: Q13263
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 21173.77

構造登録者

Zeng, L.,Yap, K.L.,Ivanov, A.V.,Wang, X.,Mujtaba, S.,Plotnikova, O.,Rauscher, F.J. (登録日: 2008-03-04, 公開日: 2008-05-20, 最終更新日: 2024-05-29)

主引用文献

Zeng, L.,Yap, K.L.,Ivanov, A.V.,Wang, X.,Mujtaba, S.,Plotnikova, O.,Rauscher, F.J.,Zhou, M.M.
Structural insights into human KAP1 PHD finger-bromodomain and its role in gene silencing
Nat.Struct.Mol.Biol., 15:626-633, 2008

PubMed Abstract: The tandem PHD finger-bromodomain, found in many chromatin-associated proteins, has an important role in gene silencing by the human co-repressor KRAB-associated protein 1 (KAP1). Here we report the three-dimensional solution structure of the tandem PHD finger-bromodomain of KAP1. The structure reveals a distinct scaffold unifying the two protein modules, in which the first helix, alpha(Z), of an atypical bromodomain forms the central hydrophobic core that anchors the other three helices of the bromodomain on one side and the zinc binding PHD finger on the other. A comprehensive mutation-based structure-function analysis correlating transcriptional repression, ubiquitin-conjugating enzyme 9 (UBC9) binding and SUMOylation shows that the PHD finger and the bromodomain of KAP1 cooperate as one functional unit to facilitate lysine SUMOylation, which is required for KAP1 co-repressor activity in gene silencing. These results demonstrate a previously unknown unified function for the tandem PHD finger-bromodomain as an intramolecular small ubiquitin-like modifier (SUMO) E3 ligase for transcriptional silencing.

PubMed: 18488044
DOI: 10.1038/nsmb.1416

実験手法

SOLUTION NMR