2RJX

Crystal structure of the headpiece domain of chicken villin, P61 space group

2RJX の概要

• エントリーDOI: 10.2210/pdb2rjx/pdb
• 関連するPDBエントリー: 1yu5 1yu7 1YU8 2RJV 2RJW 2RJY
• 分子名称: Villin-1 (2 entities in total)
• 機能のキーワード: helix, actin capping, actin-binding, calcium, cytoplasm, cytoskeleton, structural protein
• 由来する生物種: Gallus gallus (Chicken)
• 細胞内の位置: Cytoplasm, cytoskeleton: P02640
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 15223.33

構造登録者

Meng, J.,Mcknight, C.J. (登録日: 2007-10-16, 公開日: 2009-02-17, 最終更新日: 2023-08-30)

主引用文献

Meng, J.,McKnight, C.J.
Heterogeneity and dynamics in villin headpiece crystal structures.
Acta Crystallogr.,Sect.D, 65:470-476, 2009

PubMed Abstract: The villin headpiece domain (HP67) is the C-terminal F-actin-binding motif that confers F-actin-bundling activity to villin, a component of the actin bundles that support the brush-border microvilli. It has been investigated extensively by both experimental and theoretical measurements. Our laboratory, for example, has determined both its NMR and its crystal structures. This study presents the structures of HP67 and its pH-stabilized mutant (H41Y) in a different crystal form and space group. For both constructs, two molecules are found in each asymmetric unit in the new space group P6(1). While one of the two structures (Mol A) is structurally similar to our previously determined structure (Mol X), the other (Mol B) has significant deviations, especially in the N-terminal subdomain, where lattice contacts do not appear to contribute to the difference. In addition, the structurally most different crystal structure, Mol B, is actually closer to the averaged NMR structure. Harmonic motions, as suggested by the B-factor profiles, differ between these crystal structures; crystal structures from the same space group share a similar pattern. Thus, heterogeneity and dynamics are observed in different crystal structures of the same protein even for a protein as small as villin headpiece.

PubMed: 19390152
DOI: 10.1107/S0907444909008646

実験手法

X-RAY DIFFRACTION (1.7 Å)