2RCV

Crystal structure of the Bacillus subtilis superoxide dismutase

2RCV の概要

• エントリーDOI: 10.2210/pdb2rcv/pdb
• 分子名称: Superoxide dismutase [Mn], MANGANESE (II) ION (3 entities in total)
• 機能のキーワード: bacillus subtilis, superoxide dismutase, manganese, metal-binding, oxidoreductase, phosphorylation, stress response
• 由来する生物種: Bacillus subtilis
• 細胞内の位置: Cytoplasm : P54375
• タンパク質・核酸の鎖数: 8
• 化学式量合計: 181239.41

構造登録者

Liu, P.,Ewis, H.E.,Huang, Y.J.,Lu, C.D.,Tai, P.C.,Weber, I.T. (登録日: 2007-09-20, 公開日: 2008-02-26, 最終更新日: 2023-08-30)

主引用文献

Liu, P.,Ewis, H.E.,Huang, Y.J.,Lu, C.D.,Tai, P.C.,Weber, I.T.
Structure of Bacillus subtilis superoxide dismutase.
Acta Crystallogr.,Sect.F, 63:1003-1007, 2007

PubMed Abstract: The sodA gene of Bacillus subtilis was expressed in Escherichia coli, purified and crystallized. The crystal structure of MnSOD was solved by molecular replacement with four dimers per asymmetric unit and refined to an R factor of 21.1% at 1.8 A resolution. The dimer structure is very similar to that of the related enzyme from B. anthracis. Larger structural differences were observed with the human MnSOD, which has one less helix in the helical domain and a longer loop between two beta-strands and also showed differences in three amino acids at the intersubunit interface in the dimer compared with the two bacterial MnSODs. These structural differences can be exploited in the design of drugs that selectively target the Bacillus enzymes.

PubMed: 18084079
DOI: 10.1107/S1744309107054127

実験手法

X-RAY DIFFRACTION (1.6 Å)