2RCF

Carboxysome Shell protein, OrfA from H. Neapolitanus

2RCF の概要

• エントリーDOI: 10.2210/pdb2rcf/pdb
• 分子名称: Unidentified carboxysome polypeptide, CHLORIDE ION, GLYCEROL, ... (4 entities in total)
• 機能のキーワード: cyclic pentamer, structural protein
• 由来する生物種: Halothiobacillus neapolitanus
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 50168.93

構造登録者

Kerfeld, C.A.,Sawaya, M.R.,Yeates, T.O. (登録日: 2007-09-19, 公開日: 2008-04-08, 最終更新日: 2023-08-30)

主引用文献

Tanaka, S.,Kerfeld, C.A.,Sawaya, M.R.,Cai, F.,Heinhorst, S.,Cannon, G.C.,Yeates, T.O.
Atomic-level models of the bacterial carboxysome shell.
Science, 319:1083-1086, 2008

PubMed Abstract: The carboxysome is a bacterial microcompartment that functions as a simple organelle by sequestering enzymes involved in carbon fixation. The carboxysome shell is roughly 800 to 1400 angstroms in diameter and is assembled from several thousand protein subunits. Previous studies have revealed the three-dimensional structures of hexameric carboxysome shell proteins, which self-assemble into molecular layers that most likely constitute the facets of the polyhedral shell. Here, we report the three-dimensional structures of two proteins of previously unknown function, CcmL and OrfA (or CsoS4A), from the two known classes of carboxysomes, at resolutions of 2.4 and 2.15 angstroms. Both proteins assemble to form pentameric structures whose size and shape are compatible with formation of vertices in an icosahedral shell. Combining these pentamers with the hexamers previously elucidated gives two plausible, preliminary atomic models for the carboxysome shell.

PubMed: 18292340
DOI: 10.1126/science.1151458

実験手法

X-RAY DIFFRACTION (2.15 Å)