2RAI

The PX-BAR membrane remodeling unit of Sorting Nexin 9

2RAI の概要

• エントリーDOI: 10.2210/pdb2rai/pdb
• 関連するPDBエントリー: 2RAJ 2RAK
• 分子名称: Sorting nexin-9 (1 entity in total)
• 機能のキーワード: sorting nexin, membrane transport, px domain, bar domain, tubulation, structural protein
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side: Q9Y5X1
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 91907.09

構造登録者

Pylypenko, O.,Lundmark, R.,Rasmuson, E.,Carlsson, S.R.,Rak, A. (登録日: 2007-09-16, 公開日: 2007-12-11, 最終更新日: 2024-10-09)

主引用文献

Pylypenko, O.,Lundmark, R.,Rasmuson, E.,Carlsson, S.R.,Rak, A.
The PX-BAR membrane-remodeling unit of sorting nexin 9
Embo J., 26:4788-4800, 2007

PubMed Abstract: Sorting nexins (SNXs) form a family of proteins known to interact with components in the endosomal system and to regulate various steps of vesicle transport. Sorting nexin 9 (SNX9) is involved in the late stages of clathrin-mediated endocytosis in non-neuronal cells, where together with the GTPase dynamin, it participates in the formation and scission of the vesicle neck. We report here crystal structures of the functional membrane-remodeling unit of SNX9 and show that it efficiently tubulates lipid membranes in vivo and in vitro. Elucidation of the protein superdomain structure, together with mutational analysis and biochemical and cell biological experiments, demonstrated how the SNX9 PX and BAR domains work in concert in targeting and tubulation of phosphoinositide-containing membranes. The study provides insights into the SNX9-induced membrane modulation mechanism.

PubMed: 17948057
DOI: 10.1038/sj.emboj.7601889

実験手法

X-RAY DIFFRACTION (3.2 Å)