2R7R

Crystal Structure of Rotavirus SA11 VP1/RNA (UGUGACC) complex

2R7R の概要

• エントリーDOI: 10.2210/pdb2r7r/pdb
• 関連するPDBエントリー: 2R7O 2R7Q 2R7S 2R7T 2R7U 2R7V 2R7W 2R7X
• 分子名称: RNA (5'-R(*UP*GP*UP*GP*AP*CP*C)-3'), RNA-dependent RNA polymerase (2 entities in total)
• 機能のキーワード: viral protein, rna-dependent rna polymerase, single subunit polymerase fold, fingers, palm, thumb, right hand configuration, rna-directed rna polymerase, transferase-rna complex, transferase/rna
• 由来する生物種: Simian rotavirus
• 細胞内の位置: Virion : O37061
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 128521.81

構造登録者

Lu, X.,Harrison, S.C.,Tao, Y.J.,Patton, J.T.,Nibert, M.L. (登録日: 2007-09-09, 公開日: 2008-07-29, 最終更新日: 2024-02-21)

主引用文献

Lu, X.,McDonald, S.M.,Tortorici, M.A.,Tao, Y.J.,Vasquez-Del Carpio, R.,Nibert, M.L.,Patton, J.T.,Harrison, S.C.
Mechanism for coordinated RNA packaging and genome replication by rotavirus polymerase VP1.
Structure, 16:1678-1688, 2008

PubMed Abstract: Rotavirus RNA-dependent RNA polymerase VP1 catalyzes RNA synthesis within a subviral particle. This activity depends on core shell protein VP2. A conserved sequence at the 3' end of plus-strand RNA templates is important for polymerase association and genome replication. We have determined the structure of VP1 at 2.9 A resolution, as apoenzyme and in complex with RNA. The cage-like enzyme is similar to reovirus lambda3, with four tunnels leading to or from a central, catalytic cavity. A distinguishing characteristic of VP1 is specific recognition, by conserved features of the template-entry channel, of four bases, UGUG, in the conserved 3' sequence. Well-defined interactions with these bases position the RNA so that its 3' end overshoots the initiating register, producing a stable but catalytically inactive complex. We propose that specific 3' end recognition selects rotavirus RNA for packaging and that VP2 activates the autoinhibited VP1/RNA complex to coordinate packaging and genome replication.

PubMed: 19000820
DOI: 10.1016/j.str.2008.09.006

実験手法

X-RAY DIFFRACTION (2.6 Å)