2R6S

Crystal structure of Gab protein

2R6S の概要

• エントリーDOI: 10.2210/pdb2r6s/pdb
• 分子名称: Gab protein, FE (II) ION, SULFATE ION, ... (6 entities in total)
• 機能のキーワード: jelly-roll motif, unknown function
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39859.52

構造登録者

Lohkamp, B.,Dobritzsch, D. (登録日: 2007-09-06, 公開日: 2008-06-03, 最終更新日: 2023-09-20)

主引用文献

Lohkamp, B.,Dobritzsch, D.
A mixture of fortunes: the curious determination of the structure of Escherichia coli BL21 Gab protein.
Acta Crystallogr.,Sect.D, 64:407-415, 2008

PubMed Abstract: In protein crystallography, monodisperse protein samples of high purity are usually required in order to obtain diffraction-quality crystals. Here, crystals were reproducibly grown from a protein sample before its homogeneity had been determined. The sample was obtained after the first attempt to purify a recombinant target protein from an Escherichia coli cell lysate. Subsequent analysis revealed that it was a mixture of about 50 different proteins with no predominant species. Diffraction data were collected to 2.1 A and the space group was identified as I422. A molecular-replacement search with models of the expected target did not give a solution, which suggested that a contaminating E. coli protein had been crystallized. A PDB search revealed 256 structures determined in space group I422, of which 14 are E. coli proteins and two have unit-cell parameters similar to those observed. Molecular replacement with these structures showed a clear solution for one of them, the Gab protein. The structure is presented and compared with the deposited structure, from which it shows small but significant differences. The refined model contains bicine and sulfate as bound ligands, which provide insights into possible substrate-binding sites.

PubMed: 18391407
DOI: 10.1107/S0907444908001091

実験手法

X-RAY DIFFRACTION (2.1 Å)