2R6C

Crystal Form BH2

Summary for 2R6C

• Entry DOI: 10.2210/pdb2r6c/pdb
• Related: 2R6A 2R6D 2R6E
• Descriptor: Replicative helicase, DnaG Primase, Helicase Binding Domain (2 entities in total)
• Functional Keywords: helicase, primase, replication, dnab, dnag
• Biological source: Bacillus stearothermophilus; More
• Total number of polymer chains: 9
• Total formula weight: 354586.14

Authors

Bailey, S.,Eliason, W.K.,Steitz, T.A. (deposition date: 2007-09-05, release date: 2008-06-10, Last modification date: 2024-11-13)

Primary citation

Bailey, S.,Eliason, W.K.,Steitz, T.A.
Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase
Science, 318:459-463, 2007

PubMed Abstract: The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site.

PubMed: 17947583
DOI: 10.1126/science.1147353

Experimental method

X-RAY DIFFRACTION (4 Å)