2R6C

Crystal Form BH2

2R6C の概要

• エントリーDOI: 10.2210/pdb2r6c/pdb
• 関連するPDBエントリー: 2R6A 2R6D 2R6E
• 分子名称: Replicative helicase, DnaG Primase, Helicase Binding Domain (2 entities in total)
• 機能のキーワード: helicase, primase, replication, dnab, dnag
• 由来する生物種: Bacillus stearothermophilus; 詳細
• タンパク質・核酸の鎖数: 9
• 化学式量合計: 354586.14

構造登録者

Bailey, S.,Eliason, W.K.,Steitz, T.A. (登録日: 2007-09-05, 公開日: 2008-06-10, 最終更新日: 2024-11-13)

主引用文献

Bailey, S.,Eliason, W.K.,Steitz, T.A.
Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase
Science, 318:459-463, 2007

PubMed Abstract: The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site.

PubMed: 17947583
DOI: 10.1126/science.1147353

実験手法

X-RAY DIFFRACTION (4 Å)