2R6A

Crystal Form BH1

Summary for 2R6A

Entry DOI	10.2210/pdb2r6a/pdb
Related	2R6C 2R6D 2R6E
Descriptor	Replicative helicase, DnaG Primase, Helicase Binding Domain, SULFATE ION, ... (4 entities in total)
Functional Keywords	helicase, primase, replication, dnab, dnag
Biological source	Geobacillus stearothermophilus More
Total number of polymer chains	3
Total formula weight	118579.63
Authors	Bailey, S.,Eliason, W.K.,Steitz, T.A. (deposition date: 2007-09-05, release date: 2007-11-06, Last modification date: 2011-07-13)
Primary citation	Bailey, S.,Eliason, W.K.,Steitz, T.A. Structure of hexameric DnaB helicase and its complex with a domain of DnaG primase Science, 318:459-463, 2007 Cited by PubMed Abstract: The complex between the DnaB helicase and the DnaG primase unwinds duplex DNA at the eubacterial replication fork and synthesizes the Okazaki RNA primers. The crystal structures of hexameric DnaB and its complex with the helicase binding domain (HBD) of DnaG reveal that within the hexamer the two domains of DnaB pack with strikingly different symmetries to form a distinct two-layered ring structure. Each of three bound HBDs stabilizes the DnaB hexamer in a conformation that may increase its processivity. Three positive, conserved electrostatic patches on the N-terminal domain of DnaB may also serve as a binding site for DNA and thereby guide the DNA to a DnaG active site. PubMed: 17947583 DOI: 10.1126/science.1147353 PDB entries with the same primary citation
Experimental method	X-RAY DIFFRACTION (2.9 Å)

Structure validation