2R5I

Pentamer Structure of Major Capsid Protein L1 of Human Papilloma Virus type 18

2R5I の概要

• エントリーDOI: 10.2210/pdb2r5i/pdb
• 関連するPDBエントリー: 2R5H 2R5J 2R5K
• 分子名称: L1 protein (1 entity in total)
• 機能のキーワード: hpv18, pentamer, type specific epitope, capsid, capsid protein, virion, viral protein
• 由来する生物種: Human papillomavirus type 18; 詳細
• 細胞内の位置: Host nucleus : Q80B70
• タンパク質・核酸の鎖数: 15
• 化学式量合計: 713867.82

構造登録者

Bishop, B.,Dasgupta, J.,Chen, X.S. (登録日: 2007-09-03, 公開日: 2008-05-06, 最終更新日: 2024-02-21)

主引用文献

Bishop, B.,Dasgupta, J.,Klein, M.,Garcea, R.L.,Christensen, N.D.,Zhao, R.,Chen, X.S.
Crystal structures of four types of human papillomavirus L1 capsid proteins: understanding the specificity of neutralizing monoclonal antibodies.
J.Biol.Chem., 282:31803-31811, 2007

PubMed Abstract: Human papillomaviruses (HPVs) are known etiologic agents of cervical cancer. Vaccines that contain virus-like particles (VLPs) made of L1 capsid protein from several high risk HPV types have proven to be effective against HPV infections. Raising high levels of neutralizing antibodies against each HPV type is believed to be the primary mechanism of protection, gained by vaccination. Antibodies elicited by a particular HPV type are highly specific to that particular HPV type and show little or no cross-reactivity between HPV types. With an intention to understand the interplay between the L1 structure of different HPV types and the type specificity of neutralizing antibodies, we have prepared the L1 pentamers of four different HPV types, HPV11, HPV16, HPV18, and HPV35. The pentamers only bind the type-specific neutralizing monoclonal antibodies (NmAbs) that are raised against the VLP of the corresponding HPV type, implying that the surface loop structures of the pentamers from each type are distinctive and functionally active as VLPs in terms of antibody binding. We have determined the crystal structures of all four L1 pentamers, and their comparisons revealed characteristic conformational differences of the surface loops that contain the known epitopes for the NmAbs. On the basis of these distinct surface loop structures, we have provided a molecular explanation for the type specificity of NmAbs against HPV infection.

PubMed: 17804402
DOI: 10.1074/jbc.M706380200

実験手法

X-RAY DIFFRACTION (3.4 Å)