2QYU

Crystal structure of Salmonella effector protein SopA

Summary for 2QYU

• Entry DOI: 10.2210/pdb2qyu/pdb
• Related: 2QZA
• Descriptor: Secreted effector protein, PHOSPHATE ION, 2-[3-(2-HYDROXY-1,1-DIHYDROXYMETHYL-ETHYLAMINO)-PROPYLAMINO]-2-HYDROXYMETHYL-PROPANE-1,3-DIOL, ... (4 entities in total)
• Functional Keywords: ubiquitin e3 ligase, ligase
• Biological source: Salmonella typhimurium
• Cellular location: Secreted: Q8ZNR3
• Total number of polymer chains: 1
• Total formula weight: 70472.53

Authors

Diao, J.,Chen, J. (deposition date: 2007-08-15, release date: 2007-12-11, Last modification date: 2024-04-03)

Primary citation

Diao, J.,Zhang, Y.,Huibregtse, J.M.,Zhou, D.,Chen, J.
Crystal structure of SopA, a Salmonella effector protein mimicking a eukaryotic ubiquitin ligase.
Nat.Struct.Mol.Biol., 15:65-70, 2008

PubMed Abstract: Bacterial pathogens deliver virulence proteins into host cells to facilitate entry and survival. Salmonella SopA functions as an E3 ligase to manipulate the host proinflammatory response. Here we report the crystal structure of SopA in two conformations. Although it has little sequence similarity to eukaryotic HECT-domain E3s, the C-terminal half of SopA has a bilobal architecture that is reminiscent of the N- and C-lobe arrangement of HECT domains. The SopA structure also contains a putative substrate-binding domain located near the E2-binding site. The two structures of SopA differ in the relative orientations of the C lobe, indicating that SopA possesses the conformational flexibility essential for HECT E3 function. These results suggest that SopA is a unique HECT E3 ligase evolved from the coevolutionary selective pressure at the bacterium-host interface.

PubMed: 18066077
DOI: 10.1038/nsmb1346

Experimental method

X-RAY DIFFRACTION (2.1 Å)