2QWY

SAM-II riboswitch bound to S-adenosylmethionine

2QWY の概要

• エントリーDOI: 10.2210/pdb2qwy/pdb
• 分子名称: SAM-II riboswitch, PHOSPHATE ION, CESIUM ION, ... (6 entities in total)
• 機能のキーワード: mrna, riboswitch, sam, s-adenosylmethionine, adomet, rna-ligand complex, double helix, pseudoknot, base triple, rna
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 52518.38

構造登録者

Gilbert, S.D.,Rambo, R.P.,Van Tyne, D.,Batey, R.T. (登録日: 2007-08-10, 公開日: 2008-01-22, 最終更新日: 2024-02-21)

主引用文献

Gilbert, S.D.,Rambo, R.P.,Van Tyne, D.,Batey, R.T.
Structure of the SAM-II riboswitch bound to S-adenosylmethionine.
Nat.Struct.Mol.Biol., 15:177-182, 2008

PubMed Abstract: In bacteria, numerous genes harbor regulatory elements in the 5' untranslated regions of their mRNA, termed riboswitches, which control gene expression by binding small-molecule metabolites. These sequences influence the secondary and tertiary structure of the RNA in a ligand-dependent manner, thereby directing its transcription or translation. The crystal structure of an S-adenosylmethionine-responsive riboswitch found predominantly in proteobacteria, SAM-II, has been solved to reveal a second means by which RNA interacts with this important cellular metabolite. Notably, this is the first structure of a complete riboswitch containing all sequences associated with both the ligand binding aptamer domain and the regulatory expression platform. Chemical probing of this RNA in the absence and presence of ligand shows how the structure changes in response to S-adenosylmethionine to sequester the ribosomal binding site and affect translational gene regulation.

PubMed: 18204466
DOI: 10.1038/nsmb.1371

実験手法

X-RAY DIFFRACTION (2.8 Å)