2QVW
Structure of Giardia Dicer refined against twinned data
Summary for 2QVW
| Entry DOI | 10.2210/pdb2qvw/pdb |
| Related | 2FFL |
| Descriptor | GLP_546_48378_50642, MANGANESE (II) ION (2 entities in total) |
| Functional Keywords | dicer, rnai, pseudo-merohedral twin, hydrolase |
| Biological source | Giardia intestinalis |
| Total number of polymer chains | 4 |
| Total formula weight | 332902.86 |
| Authors | Doudna, J.A.,MacRae, I.J. (deposition date: 2007-08-09, release date: 2007-09-25, Last modification date: 2023-08-30) |
| Primary citation | Macrae, I.J.,Doudna, J.A. An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer Acta Crystallogr.,Sect.D, 63:993-999, 2007 Cited by PubMed Abstract: The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map. PubMed: 17704568DOI: 10.1107/S0907444907036128 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (3 Å) |
Structure validation
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