2QVW

Structure of Giardia Dicer refined against twinned data

2QVW の概要

• エントリーDOI: 10.2210/pdb2qvw/pdb
• 関連するPDBエントリー: 2FFL
• 分子名称: GLP_546_48378_50642, MANGANESE (II) ION (2 entities in total)
• 機能のキーワード: dicer, rnai, pseudo-merohedral twin, hydrolase
• 由来する生物種: Giardia intestinalis
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 332902.86

構造登録者

Doudna, J.A.,MacRae, I.J. (登録日: 2007-08-09, 公開日: 2007-09-25, 最終更新日: 2023-08-30)

主引用文献

Macrae, I.J.,Doudna, J.A.
An unusual case of pseudo-merohedral twinning in orthorhombic crystals of Dicer
Acta Crystallogr.,Sect.D, 63:993-999, 2007

PubMed Abstract: The crystal structure of the enzyme Dicer from Giardia intestinalis was solved to 3.3 A resolution by MAD using crystals belonging to space group P2(1)2(1)2 [Macrae et al. (2006), Science, 311, 195-198]. These crystals were derived from crystals that diffracted X-rays to 3.0 A resolution but were refractory to structure determination because they were twinned. It is shown here that the original Dicer crystals represent an unusual case of perfect pseudo-merohedral twinning of orthorhombic crystals. Before the twinning problem was overcome, it was possible to calculate a low-resolution electron-density map in space group P4(1) that was used to build a partial molecular model. Experimental phases were sufficient to identify heavy-atom sites that indicated space-group inconsistency, leading to identification of the true space group. This information guided the search for different crystallization conditions that yielded untwinned crystals and ultimately a fully interpretable electron-density map.

PubMed: 17704568
DOI: 10.1107/S0907444907036128

実験手法

X-RAY DIFFRACTION (3 Å)