2QU4

Model for Bacterial ParM Filament

2QU4 の概要

• エントリーDOI: 10.2210/pdb2qu4/pdb
• 分子名称: Plasmid segregation protein parM (1 entity in total)
• 機能のキーワード: filament model, actin-like protein, helical polymer, plasmid, plasmid partition, structural protein
• 由来する生物種: Escherichia coli
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 35804.38

構造登録者

Orlova, A.,Garner, E.C.,Galkin, V.E.,Heuser, J.,Mullins, R.D.,Egelman, E.H. (登録日: 2007-08-03, 公開日: 2007-09-18, 最終更新日: 2024-02-21)

主引用文献

Orlova, A.,Garner, E.C.,Galkin, V.E.,Heuser, J.,Mullins, R.D.,Egelman, E.H.
The structure of bacterial ParM filaments.
Nat.Struct.Mol.Biol., 14:921-926, 2007

PubMed Abstract: Bacterial ParM is a homolog of eukaryotic actin and is involved in moving plasmids so that they segregate properly during cell division. Using cryo-EM and three-dimensional reconstruction, we show that ParM filaments have a different structure from F-actin, with very different subunit-subunit interfaces. These interfaces result in the helical handedness of the ParM filament being opposite to that of F-actin. Like F-actin, ParM filaments have a variable twist, and we show that this involves domain-domain rotations within the ParM subunit. The present results yield new insights into polymorphisms within F-actin, as well as the evolution of polymer families.

PubMed: 17873883
DOI: 10.1038/nsmb1300

実験手法

ELECTRON MICROSCOPY (16 Å)