2QHR

Crystal structure of the 13F6-1-2 Fab fragment bound to its Ebola virus glycoprotein peptide epitope.

2QHR の概要

• エントリーDOI: 10.2210/pdb2qhr/pdb
• 分子名称: 13F6-1-2 Fab fragment heavy chain, 13F6-1-2 Fab fragment V lambda x light chain, Envelope glycoprotein peptide, ... (4 entities in total)
• 機能のキーワード: immunologlobulin fold, antibody-peptide complex, immune system-viral protein complex, immune system/viral protein
• 由来する生物種: Mus musculus (house mouse); 詳細
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 49033.51

構造登録者

Lee, J.E.,Kuehne, A.,Abelson, D.M.,Fusco, M.L.,Hart, M.K.,Saphire, E.O. (登録日: 2007-07-02, 公開日: 2008-01-22, 最終更新日: 2024-11-20)

主引用文献

Lee, J.E.,Kuehne, A.,Abelson, D.M.,Fusco, M.L.,Hart, M.K.,Saphire, E.O.
Complex of a protective antibody with its Ebola virus GP peptide epitope: unusual features of a V lambda x light chain.
J.Mol.Biol., 375:202-216, 2008

PubMed Abstract: 13F6-1-2 is a murine monoclonal antibody that recognizes the heavily glycosylated mucin-like domain of the Ebola virus virion-attached glycoprotein (GP) and protects animals against lethal viral challenge. Here we present the crystal structure, at 2.0 A, of 13F6-1-2 in complex with its Ebola virus GP peptide epitope. The GP peptide binds in an extended conformation, anchored primarily by interactions with the heavy chain. Two GP residues, Gln P406 and Arg P409, make extensive side-chain hydrogen bond and electrostatic interactions with the antibody and are likely critical for recognition and affinity. The 13F6-1-2 antibody utilizes a rare V lambda(x) light chain. The three light-chain complementarity-determining regions do not adopt canonical conformations and represent new classes of structures distinct from V kappa and other V lambda light chains. In addition, although V lambda(x) had been thought to confer specificity, all light-chain contacts are mediated through germ-line-encoded residues. This structure of an antibody that protects against the Ebola virus now provides a framework for humanization and development of a postexposure immunotherapeutic.

PubMed: 18005986
DOI: 10.1016/j.jmb.2007.10.017

実験手法

X-RAY DIFFRACTION (2 Å)