2QH2
Solution structure of the CR7 terminal hairpin loop from human telomerase RNA
Summary for 2QH2
| Entry DOI | 10.2210/pdb2qh2/pdb |
| NMR Information | BMRB: 7403 |
| Descriptor | Human telomerase RNA CR7 terminal hairpin loop (1 entity in total) |
| Functional Keywords | loop, wobble base pair, syn g, rna |
| Total number of polymer chains | 1 |
| Total formula weight | 7716.61 |
| Authors | Feigon, J.,Theimer, C.A.,Chim, N.,Breece, K.E. (deposition date: 2007-06-29, release date: 2007-09-25, Last modification date: 2024-05-01) |
| Primary citation | Theimer, C.A.,Jady, B.E.,Chim, N.,Richard, P.,Breece, K.E.,Kiss, T.,Feigon, J. Structural and functional characterization of human telomerase RNA processing and cajal body localization signals. Mol.Cell, 27:869-881, 2007 Cited by PubMed Abstract: The RNA component of human telomerase (hTR) includes H/ACA and CR7 domains required for 3' end processing, localization, and accumulation. The terminal loop of the CR7 domain contains the CAB box (ugAG) required for targeting of scaRNAs to Cajal bodies (CB) and an uncharacterized sequence required for accumulation and processing. To dissect out the contributions of the CR7 stem loop to hTR processing and localization, we solved the solution structures of the 3' terminal stem loops of hTR CR7 and U64 H/ACA snoRNA, and the 5' terminal stem loop of U85 C/D-H/ACA scaRNA. These structures, together with analysis of localization, processing, and accumulation of hTRs containing nucleotide substitutions in the CR7 domain, identified the sequence and structural requirements of the hTR processing and CB localization signals and showed that these signals are functionally independent. Further, 3' end processing was found to be a prerequisite for translocation of hTR to CBs. PubMed: 17889661DOI: 10.1016/j.molcel.2007.07.017 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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