2QDN

Crystal Structure of mouse GITRL

2QDN の概要

• エントリーDOI: 10.2210/pdb2qdn/pdb
• 分子名称: GITR ligand (2 entities in total)
• 機能のキーワード: gitrl; glucocorticoid-induced tnf receptor ligand, immune system
• 由来する生物種: Mus musculus (house mouse)
• 細胞内の位置: Cell membrane ; Single-pass type II membrane protein : Q7TS55
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 30022.81

構造登録者

Chattopadhyay, K.,Ramagopal, U.A.,Nathenson, S.G.,Almo, S.C. (登録日: 2007-06-21, 公開日: 2008-01-01, 最終更新日: 2024-11-13)

主引用文献

Chattopadhyay, K.,Ramagopal, U.A.,Brenowitz, M.,Nathenson, S.G.,Almo, S.C.
Evolution of GITRL immune function: murine GITRL exhibits unique structural and biochemical properties within the TNF superfamily.
Proc.Natl.Acad.Sci.Usa, 105:635-640, 2008

PubMed Abstract: Glucocorticoid-induced TNF receptor ligand (GITRL), a recently identified member of the TNF superfamily, binds to its receptor, GITR, on both effector and regulatory T cells and generates positive costimulatory signals implicated in a wide range of T cell functions. In contrast to all previously characterized homotrimeric TNF family members, the mouse GITRL crystal structure reveals a previously unrecognized dimeric assembly that is stabilized via a unique "domain-swapping" interaction. Consistent with its crystal structure, mouse GITRL exists as a stable dimer in solution. Structure-guided mutagenesis studies confirmed the determinants responsible for dimerization and support a previously unrecognized receptor-recognition surface for mouse GITRL that has not been observed for any other TNF family members. Taken together, the unique structural and biochemical behavior of mouse GITRL, along with the unusual domain organization of murine GITR, support a previously unrecognized mechanism for signaling within the TNF superfamily.

PubMed: 18182486
DOI: 10.1073/pnas.0710529105

実験手法

X-RAY DIFFRACTION (2.09 Å)