2QCA

A New Crystal Form of Bovine Pancreatic RNase A in Complex with 2'-Deoxyguanosine-5'-monophosphate

2QCA の概要

• エントリーDOI: 10.2210/pdb2qca/pdb
• 分子名称: Ribonuclease pancreatic, 2'-DEOXYGUANOSINE-5'-MONOPHOSPHATE (3 entities in total)
• 機能のキーワード: ribonuclease, protein-nucleotide complex, structural genomics, psi-2, protein structure initiative, center for high-throughput structural biology, chtsb, hydrolase
• 由来する生物種: Bos taurus (cattle)
• 細胞内の位置: Secreted: P61823
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 14402.77

構造登録者

Larson, S.B.,Day, J.S.,Cudney, R.,McPherson, A.,Center for High-Throughput Structural Biology (CHTSB) (登録日: 2007-06-19, 公開日: 2007-07-03, 最終更新日: 2024-11-20)

主引用文献

Larson, S.B.,Day, J.S.,Cudney, R.,McPherson, A.
A new crystal form of bovine pancreatic RNase A in complex with 2'-deoxyguanosine-5'-monophosphate.
Acta Crystallogr.,Sect.F, 63:728-733, 2007

PubMed Abstract: The structure of bovine pancreatic RNase A has been determined in complex with 2'-deoxyguanosine-5'-monophosphate (dGMP) at 1.33 A resolution at room temperature in a previously unreported unit cell belonging to space group P3(1). There are two molecules of nucleotide per enzyme molecule, one of which lies in the active-site cleft in the productive binding mode, whilst the guanine base of the other dGMP occupies the pyrimidine-specific binding site in a nonproductive mode such that it forms hydrogen bonds to the phosphate group of the first dGMP. This is the first RNase A structure containing a guanine base in the B2 binding site. Each dGMP molecule is involved in intermolecular interactions with adjacent RNase A molecules in the lattice and the two nucleotides appear to direct the formation of the crystal lattice. Because GMP may be produced during degradation of RNA, this association could represent an inhibitor complex and thereby affect the observed enzyme kinetics.

PubMed: 17768339
DOI: 10.1107/S1744309107039565

実験手法

X-RAY DIFFRACTION (1.33 Å)