2QBZ

Structure of the M-Box Riboswitch Aptamer Domain

Summary for 2QBZ

• Entry DOI: 10.2210/pdb2qbz/pdb
• Descriptor: M-Box RNA, ykoK riboswitch aptamer, MAGNESIUM ION, POTASSIUM ION, ... (4 entities in total)
• Functional Keywords: rna, riboswitch, ykok, m-box, rna sensor, noncoding rna
• Total number of polymer chains: 1
• Total formula weight: 52112.93

Authors

Dann III, C.E.,Winkler, W.C. (deposition date: 2007-06-18, release date: 2007-09-18, Last modification date: 2024-02-21)

Primary citation

Dann III, C.E.,Wakeman, C.A.,Sieling, C.L.,Baker, S.C.,Irnov, I.,Winkler, W.C.
Structure and mechanism of a metal-sensing regulatory RNA
Cell(Cambridge,Mass.), 130:878-892, 2007

PubMed Abstract: Organisms maintain the correct balance of intracellular metals primarily through metal-sensing proteins that control transport and storage of the target ion(s). Here, we reveal the basis of metal sensing and genetic control by a metalloregulatory RNA. Our data demonstrate that a previously uncharacterized orphan riboswitch, renamed the "M-box," is a divalent metal-sensing RNA involved in Mg(2+) homeostasis. A combination of genetic, biochemical, and biophysical techniques demonstrate that Mg(2+) induces a compacted tertiary architecture for M-box RNAs that regulates the accessibility of nucleotides involved in genetic control. Molecular details are provided by crystallographic structure determination of a Mg(2+)-bound M-box RNA. Given the distribution of this RNA element, it may constitute a common mode for bacterial metal ion regulation, and its discovery suggests the possibility of additional RNA-based metal sensors in modern and primordial organisms.

PubMed: 17803910
DOI: 10.1016/j.cell.2007.06.051

Experimental method

X-RAY DIFFRACTION (2.6 Å)