2Q9T

High-resolution structure of the DING protein from Pseudomonas fluorescens

Summary for 2Q9T

• Entry DOI: 10.2210/pdb2q9t/pdb
• Descriptor: DING, PHOSPHATE ION, ACETATE ION, ... (7 entities in total)
• Functional Keywords: ding protein, phosphate-binding, venus flytrap fold, psts protein, unknown function
• Biological source: Pseudomonas fluorescens
• Total number of polymer chains: 1
• Total formula weight: 39784.75

Authors

Moniot, S.,Ahn, S.,Elias, M.,Kim, D.,Scott, K.,Chabriere, E. (deposition date: 2007-06-14, release date: 2008-06-17, Last modification date: 2023-08-30)

Primary citation

Ahn, S.,Moniot, S.,Elias, M.,Chabriere, E.,Kim, D.,Scott, K.
Structure-function relationships in a bacterial DING protein.
Febs Lett., 581:3455-3460, 2007

PubMed Abstract: A recombinant DING protein from Pseudomonas fluorescens has been previously shown to have a phosphate-binding site, and to be mitogenic for human cells. Here we report the three-dimensional structure of the protein, confirming a close similarity to the "Venus flytrap" structure seen in other human and bacterial phosphate-binding proteins. Site-directed mutagenesis confirms the role of a key residue involved in phosphate binding, and that the mitogenic activity is not dependent on this property. Deletion of one of the two hinged domains that constitute the Venus flytrap also eliminates phosphate binding whilst enhancing mitogenic activity.

PubMed: 17612529
DOI: 10.1016/j.febslet.2007.06.050

Experimental method

X-RAY DIFFRACTION (1.43 Å)