2Q9T

High-resolution structure of the DING protein from Pseudomonas fluorescens

2Q9T の概要

• エントリーDOI: 10.2210/pdb2q9t/pdb
• 分子名称: DING, PHOSPHATE ION, ACETATE ION, ... (7 entities in total)
• 機能のキーワード: ding protein, phosphate-binding, venus flytrap fold, psts protein, unknown function
• 由来する生物種: Pseudomonas fluorescens
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 39784.75

構造登録者

Moniot, S.,Ahn, S.,Elias, M.,Kim, D.,Scott, K.,Chabriere, E. (登録日: 2007-06-14, 公開日: 2008-06-17, 最終更新日: 2024-11-13)

主引用文献

Ahn, S.,Moniot, S.,Elias, M.,Chabriere, E.,Kim, D.,Scott, K.
Structure-function relationships in a bacterial DING protein.
Febs Lett., 581:3455-3460, 2007

PubMed Abstract: A recombinant DING protein from Pseudomonas fluorescens has been previously shown to have a phosphate-binding site, and to be mitogenic for human cells. Here we report the three-dimensional structure of the protein, confirming a close similarity to the "Venus flytrap" structure seen in other human and bacterial phosphate-binding proteins. Site-directed mutagenesis confirms the role of a key residue involved in phosphate binding, and that the mitogenic activity is not dependent on this property. Deletion of one of the two hinged domains that constitute the Venus flytrap also eliminates phosphate binding whilst enhancing mitogenic activity.

PubMed: 17612529
DOI: 10.1016/j.febslet.2007.06.050

実験手法

X-RAY DIFFRACTION (1.43 Å)