2Q7C

Crystal structure of IQN17

2Q7C の概要

• エントリーDOI: 10.2210/pdb2q7c/pdb
• 関連するPDBエントリー: 2Q5U
• 分子名称: fusion protein between yeast variant GCN4 and HIVgp41, CHLORIDE ION (3 entities in total)
• 機能のキーワード: envelope glycoprotein, coiled coil, viral protein/viral protein inhibitor, viral protein
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 16547.51

構造登録者

Malashkevich, V.N.,Eckert, D.M.,Hong, L.H.,Kim, P.S. (登録日: 2007-06-06, 公開日: 2007-06-19, 最終更新日: 2024-10-30)

主引用文献

Eckert, D.M.,Malashkevich, V.N.,Hong, L.H.,Carr, P.A.,Kim, P.S.
Inhibiting HIV Entry: Discovery of D-Peptide Inhibitors that Target the Gp41 Coiled-Coil Pocket
Cell(Cambridge,Mass.), 99:103-105, 1999

PubMed Abstract: The HIV-1 gp41 protein promotes viral entry by mediating the fusion of viral and cellular membranes. A prominent pocket on the surface of a central trimeric coiled coil within gp41 was previously identified as a potential target for drugs that inhibit HIV-1 entry. We designed a peptide, IQN17, which properly presents this pocket. Utilizing IQN17 and mirror-image phage display, we identified cyclic, D-peptide inhibitors of HIV-1 infection that share a sequence motif. A 1.5 A cocrystal structure of IQN17 in complex with a D-peptide, and NMR studies, show that conserved residues of these inhibitors make intimate contact with the gp41 pocket. Our studies validate the pocket per se as a target for drug development. IQN17 and these D-peptide inhibitors are likely to be useful for development and identification of a new class of orally bioavailable anti-HIV drugs.

PubMed: 10520998
DOI: 10.1016/S0092-8674(00)80066-5

実験手法

X-RAY DIFFRACTION (2 Å)