Loading
PDBj
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

2Q4J

Ensemble refinement of the protein crystal structure of gene product from Arabidopsis thaliana At3g03250, a putative UDP-glucose pyrophosphorylase

Summary for 2Q4J
Entry DOI10.2210/pdb2q4j/pdb
Related2ICX 2ICY
DescriptorProbable UTP-glucose-1-phosphate uridylyltransferase 2 (2 entities in total)
Functional Keywordsensemble refinement, refinement methodology development, at3g03250, putative udp-glucose pyrophosphorylase, structural genomics, protein structure initiative, psi, center for eukaryotic structural genomics, cesg, transferase
Biological sourceArabidopsis thaliana (thale cress)
Cellular locationCytoplasm (By similarity): Q9M9P3
Total number of polymer chains2
Total formula weight103596.41
Authors
Levin, E.J.,Kondrashov, D.A.,Wesenberg, G.E.,Phillips Jr., G.N.,Center for Eukaryotic Structural Genomics (CESG) (deposition date: 2007-05-31, release date: 2007-06-19, Last modification date: 2023-08-30)
Primary citationLevin, E.J.,Kondrashov, D.A.,Wesenberg, G.E.,Phillips, G.N.
Ensemble refinement of protein crystal structures: validation and application.
Structure, 15:1040-1052, 2007
Cited by
PubMed Abstract: X-ray crystallography typically uses a single set of coordinates and B factors to describe macromolecular conformations. Refinement of multiple copies of the entire structure has been previously used in specific cases as an alternative means of representing structural flexibility. Here, we systematically validate this method by using simulated diffraction data, and we find that ensemble refinement produces better representations of the distributions of atomic positions in the simulated structures than single-conformer refinements. Comparison of principal components calculated from the refined ensembles and simulations shows that concerted motions are captured locally, but that correlations dissipate over long distances. Ensemble refinement is also used on 50 experimental structures of varying resolution and leads to decreases in R(free) values, implying that improvements in the representation of flexibility observed for the simulated structures may apply to real structures. These gains are essentially independent of resolution or data-to-parameter ratio, suggesting that even structures at moderate resolution can benefit from ensemble refinement.
PubMed: 17850744
DOI: 10.1016/j.str.2007.06.019
PDB entries with the same primary citation
Experimental method
X-RAY DIFFRACTION (1.863 Å)
Structure validation

227561

数据于2024-11-20公开中

PDB statisticsPDBj update infoContact PDBjnumon