2Q2U

Structure of Chlorella virus DNA ligase-product DNA complex

2Q2U の概要

• エントリーDOI: 10.2210/pdb2q2u/pdb
• 関連するPDBエントリー: 1FVI 2Q2T
• 分子名称: 5'-D(*TP*TP*CP*CP*GP*AP*TP*AP*GP*TP*GP*GP*GP*GP*TP*CP*GP*CP*AP*AP*T)-3', 5'-D(*AP*TP*TP*GP*CP*GP*AP*CP*(OMC)P*CP*CP*AP*CP*TP*AP*TP*CP*GP*GP*AP*A)-3', Chlorella virus DNA ligase, ... (4 entities in total)
• 機能のキーワード: ligase, product-complex, protein-dna complex, ligase-dna complex, ligase/dna
• 由来する生物種: Paramecium bursaria Chlorella virus 1; 詳細
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 198997.95

構造登録者

Lima, C.D.,Nandakumar, J.,Nair, P.A.,Smith, P.,Shuman, S. (登録日: 2007-05-29, 公開日: 2007-07-10, 最終更新日: 2024-04-03)

主引用文献

Nair, P.A.,Nandakumar, J.,Smith, P.,Odell, M.,Lima, C.D.,Shuman, S.
Structural basis for nick recognition by a minimal pluripotent DNA ligase.
Nat.Struct.Mol.Biol., 14:770-778, 2007

PubMed Abstract: Chlorella virus DNA ligase, the smallest eukaryotic ligase known, has pluripotent biological activity and an intrinsic nick-sensing function, despite having none of the accessory domains found in cellular ligases. A 2.3-A crystal structure of the Chlorella virus ligase-AMP intermediate bound to duplex DNA containing a 3'-OH-5'-PO4 nick reveals a new mode of DNA envelopment, in which a short surface loop emanating from the OB domain forms a beta-hairpin 'latch' that inserts into the DNA major groove flanking the nick. A network of interactions with the 3'-OH and 5'-PO4 termini in the active site illuminates the DNA adenylylation mechanism and the crucial roles of AMP in nick sensing and catalysis. Addition of a divalent cation triggered nick sealing in crystallo, establishing that the nick complex is a bona fide intermediate in the DNA repair pathway.

PubMed: 17618295
DOI: 10.1038/nsmb1266

実験手法

X-RAY DIFFRACTION (3 Å)