2Q0S
Structure of the Inhibitor bound form of M. Smegmatis Aryl Esterase
Summary for 2Q0S
| Entry DOI | 10.2210/pdb2q0s/pdb |
| Related | 2q0q |
| Descriptor | Aryl esterase, SULFATE ION (3 entities in total) |
| Functional Keywords | sgnh hydrolase, oligomeric enzyme, acyl transfer, aryl esterase, covalent inhibitor, hydrolase |
| Biological source | Mycobacterium smegmatis |
| Total number of polymer chains | 8 |
| Total formula weight | 187662.42 |
| Authors | Mathews, I.I.,Soltis, M.,Saldajeno, M.,Ganshaw, G.,Sala, R.,Weyler, W.,Cervin, M.A.,Whited, G.,Bott, R. (deposition date: 2007-05-22, release date: 2007-12-11, Last modification date: 2024-04-03) |
| Primary citation | Mathews, I.,Soltis, M.,Saldajeno, M.,Ganshaw, G.,Sala, R.,Weyler, W.,Cervin, M.A.,Whited, G.,Bott, R. Structure of a novel enzyme that catalyzes acyl transfer to alcohols in aqueous conditions. Biochemistry, 46:8969-8979, 2007 Cited by PubMed Abstract: The unusual architecture of the enzyme (MsAcT) isolated from Mycobacterium smegmatis forms the mechanistic basis for favoring alcoholysis over hydrolysis in water. Unlike hydrolases that perform alcoholysis only under anhydrous conditions, MsAcT demonstrates alcoholysis in substantially aqueous media and, in the presence of hydrogen peroxide, has a perhydrolysis:hydrolysis ratio 50-fold greater than that of the best lipase tested. The crystal structures of the apoenzyme and an inhibitor-bound form have been determined to 1.5 A resolution. MsAcT is an octamer in the asymmetric unit and forms a tightly associated aggregate in solution. Relative to other structurally similar monomers, MsAcT contains several insertions that contribute to the oligomerization and greatly restrict the shape of the active site, thereby limiting its accessibility. These properties create an environment by which MsAcT can catalyze transesterification reactions in an aqueous medium and suggests how a serine hydrolase can be engineered to be an efficient acyltransferase. PubMed: 17636869DOI: 10.1021/bi7002444 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (1.5 Å) |
Structure validation
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