2PZ9

Crystal structure of putative transcriptional regulator SCO4942 from Streptomyces coelicolor

Summary for 2PZ9

• Entry DOI: 10.2210/pdb2pz9/pdb
• Descriptor: Putative regulatory protein, SULFATE ION (3 entities in total)
• Functional Keywords: structural genomics, transcriptional regulator, streptomyces coelicolor a3(2), psi, protein structure initiative, midwest center for structural genomics, mcsg, transcription
• Biological source: Streptomyces coelicolor A3(2)
• Total number of polymer chains: 1
• Total formula weight: 25120.45

Authors

Filippova, E.V.,Chruszcz, M.,Xu, X.,Zheng, H.,Cymborowski, M.,Savchenko, A.,Edwards, A.,Joachimiak, A.,Minor, W.,Midwest Center for Structural Genomics (MCSG) (deposition date: 2007-05-17, release date: 2007-06-19, Last modification date: 2024-10-30)

Primary citation

Dong, A.,Xu, X.,Edwards, A.M.,Chang, C.,Chruszcz, M.,Cuff, M.,Cymborowski, M.,Di Leo, R.,Egorova, O.,Evdokimova, E.,Filippova, E.,Gu, J.,Guthrie, J.,Ignatchenko, A.,Joachimiak, A.,Klostermann, N.,Kim, Y.,Korniyenko, Y.,Minor, W.,Que, Q.,Savchenko, A.,Skarina, T.,Tan, K.,Yakunin, A.,Yee, A.,Yim, V.,Zhang, R.,Zheng, H.,Akutsu, M.,Arrowsmith, C.,Avvakumov, G.V.,Bochkarev, A.,Dahlgren, L.G.,Dhe-Paganon, S.,Dimov, S.,Dombrovski, L.,Finerty, P.,Flodin, S.,Flores, A.,Graslund, S.,Hammerstrom, M.,Herman, M.D.,Hong, B.S.,Hui, R.,Johansson, I.,Liu, Y.,Nilsson, M.,Nedyalkova, L.,Nordlund, P.,Nyman, T.,Min, J.,Ouyang, H.,Park, H.W.,Qi, C.,Rabeh, W.,Shen, L.,Shen, Y.,Sukumard, D.,Tempel, W.,Tong, Y.,Tresagues, L.,Vedadi, M.,Walker, J.R.,Weigelt, J.,Welin, M.,Wu, H.,Xiao, T.,Zeng, H.,Zhu, H.
In situ proteolysis for protein crystallization and structure determination.
Nat.Methods, 4:1019-1021, 2007

PubMed Abstract: We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

PubMed: 17982461
DOI: 10.1038/nmeth1118

Experimental method

X-RAY DIFFRACTION (2.8 Å)