2PZ9

Crystal structure of putative transcriptional regulator SCO4942 from Streptomyces coelicolor

2PZ9 の概要

• エントリーDOI: 10.2210/pdb2pz9/pdb
• 分子名称: Putative regulatory protein, SULFATE ION (3 entities in total)
• 機能のキーワード: structural genomics, transcriptional regulator, streptomyces coelicolor a3(2), psi, protein structure initiative, midwest center for structural genomics, mcsg, transcription
• 由来する生物種: Streptomyces coelicolor A3(2)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 25120.45

構造登録者

Filippova, E.V.,Chruszcz, M.,Xu, X.,Zheng, H.,Cymborowski, M.,Savchenko, A.,Edwards, A.,Joachimiak, A.,Minor, W.,Midwest Center for Structural Genomics (MCSG) (登録日: 2007-05-17, 公開日: 2007-06-19, 最終更新日: 2024-10-30)

主引用文献

Dong, A.,Xu, X.,Edwards, A.M.,Chang, C.,Chruszcz, M.,Cuff, M.,Cymborowski, M.,Di Leo, R.,Egorova, O.,Evdokimova, E.,Filippova, E.,Gu, J.,Guthrie, J.,Ignatchenko, A.,Joachimiak, A.,Klostermann, N.,Kim, Y.,Korniyenko, Y.,Minor, W.,Que, Q.,Savchenko, A.,Skarina, T.,Tan, K.,Yakunin, A.,Yee, A.,Yim, V.,Zhang, R.,Zheng, H.,Akutsu, M.,Arrowsmith, C.,Avvakumov, G.V.,Bochkarev, A.,Dahlgren, L.G.,Dhe-Paganon, S.,Dimov, S.,Dombrovski, L.,Finerty, P.,Flodin, S.,Flores, A.,Graslund, S.,Hammerstrom, M.,Herman, M.D.,Hong, B.S.,Hui, R.,Johansson, I.,Liu, Y.,Nilsson, M.,Nedyalkova, L.,Nordlund, P.,Nyman, T.,Min, J.,Ouyang, H.,Park, H.W.,Qi, C.,Rabeh, W.,Shen, L.,Shen, Y.,Sukumard, D.,Tempel, W.,Tong, Y.,Tresagues, L.,Vedadi, M.,Walker, J.R.,Weigelt, J.,Welin, M.,Wu, H.,Xiao, T.,Zeng, H.,Zhu, H.
In situ proteolysis for protein crystallization and structure determination.
Nat.Methods, 4:1019-1021, 2007

PubMed Abstract: We tested the general applicability of in situ proteolysis to form protein crystals suitable for structure determination by adding a protease (chymotrypsin or trypsin) digestion step to crystallization trials of 55 bacterial and 14 human proteins that had proven recalcitrant to our best efforts at crystallization or structure determination. This is a work in progress; so far we determined structures of 9 bacterial proteins and the human aminoimidazole ribonucleotide synthetase (AIRS) domain.

PubMed: 17982461
DOI: 10.1038/nmeth1118

実験手法

X-RAY DIFFRACTION (2.8 Å)