2PYY

Crystal Structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with (L)-glutamate

Summary for 2PYY

• Entry DOI: 10.2210/pdb2pyy/pdb
• Descriptor: Ionotropic glutamate receptor bacterial homologue, GLUTAMIC ACID (3 entities in total)
• Functional Keywords: glur0 ligand binding domain, transport protein
• Biological source: Nostoc punctiforme
• Total number of polymer chains: 3
• Total formula weight: 75707.23

Authors

Lee, J.H.,Kang, G.B.,Lim, H.-H.,Ree, M.,Park, C.-S.,Eom, S.H. (deposition date: 2007-05-17, release date: 2008-01-22, Last modification date: 2023-08-30)

Primary citation

Lee, J.H.,Kang, G.B.,Lim, H.H.,Jin, K.S.,Kim, S.H.,Ree, M.,Park, C.S.,Kim, S.J.,Eom, S.H.
Crystal structure of the GluR0 ligand-binding core from Nostoc punctiforme in complex with L-glutamate: structural dissection of the ligand interaction and subunit interface.
J.Mol.Biol., 376:308-316, 2008

PubMed Abstract: GluR0 from Nostoc punctiforme (NpGluR0) is a bacterial homologue of the ionotropic glutamate receptor (iGluR). We have solved the crystal structure of the ligand-binding core of NpGluR0 in complex with l-glutamate at a resolution of 2.1 A. The structure exhibits a noncanonical ligand interaction and two distinct subunit interfaces. The side-chain guanidium group of Arg80 forms a salt bridge with the gamma-carboxyl group of bound L-glutamate: in GluR0 from Synechocystis (SGluR0) and other iGluRs, the equivalent residues are Asn or Thr, which cannot form a similar interaction. We suggest that the local positively charged environment and the steric constraint created by Arg80 mediate the selectivity of L-glutamate binding by preventing the binding of positively charged and hydrophobic amino acids. In addition, the NpGluR0 ligand-binding core forms a new subunit interface in which the two protomers are arranged differently than the known iGluR and SGluR0 dimer interfaces. The significance of there being two different dimer interfaces was investigated using analytical ultracentrifugation analysis.

PubMed: 18164033
DOI: 10.1016/j.jmb.2007.10.081

Experimental method

X-RAY DIFFRACTION (2.1 Å)