2PYA

Ultra-high resolution structure of P. abyssi rubredoxin W4L/R5S/A44S

2PYA の概要

• エントリーDOI: 10.2210/pdb2pya/pdb
• 関連するPDBエントリー: 1yk4 1yk5
• 分子名称: Rubredoxin, FE (III) ION, SODIUM ION, ... (4 entities in total)
• 機能のキーワード: o-h...s-fe hydrogen bond, electron transport
• 由来する生物種: Pyrococcus abyssi
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 5890.19

構造登録者

Boenisch, H.,Ladenstein, R. (登録日: 2007-05-16, 公開日: 2008-04-29, 最終更新日: 2024-02-21)

主引用文献

Bonisch, H.,Schmidt, C.L.,Bianco, P.,Ladenstein, R.
Ultrahigh-resolution study on Pyrococcus abyssi rubredoxin: II. Introduction of an O-H...Sgamma-Fe hydrogen bond increased the reduction potential by 65 mV.
J.Biol.Inorg.Chem., 12:1163-1171, 2007

PubMed Abstract: The effect of D-H...S(gamma)-Fe hydrogen bonding on the reduction potential of rubredoxin was investigated by the introduction of an O-H...S(gamma)-Fe hydrogen bond on the surface of Pyrococcus abyssi rubredoxin. The formation of a weak hydrogen bond between Ser44-O(gamma) and Cys42-S(gamma) in mutant W4L/R5S/A44S increased the reduction potential by 56 mV. When side effects of the mutation were taken into account, the contribution of the additional cluster hydrogen bond to the reduction potential was estimated to be +65 mV. The structural analysis was based on ultrahigh-resolution structures of oxidized P. abyssi rubredoxin W4L/R5S and W4L/R5S/A44S refined to 0.69 and 0.86 A, respectively.

PubMed: 17712580
DOI: 10.1007/s00775-007-0289-8

実験手法

X-RAY DIFFRACTION (0.86 Å)