2PXP

Variant 13 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle

2PXP の概要

• エントリーDOI: 10.2210/pdb2pxp/pdb
• 関連するPDBエントリー: 1DUL
• 分子名称: 4.5 S RNA, Signal recognition particle protein, COBALT HEXAMMINE(III) (3 entities in total)
• 機能のキーワード: gu pair, hexamine, rna phasing, rna, cation binding, signaling protein-rna complex, signaling protein/rna
• 由来する生物種: Escherichia coli; 詳細
• 細胞内の位置: Cytoplasm: P0AGD7
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 29348.99

構造登録者

Keel, A.Y.,Rambo, R.P.,Batey, R.T.,Kieft, J.S. (登録日: 2007-05-14, 公開日: 2007-08-07, 最終更新日: 2024-10-30)

主引用文献

Keel, A.Y.,Rambo, R.P.,Batey, R.T.,Kieft, J.S.
A General Strategy to Solve the Phase Problem in RNA Crystallography.
Structure, 15:761-772, 2007

PubMed Abstract: X-ray crystallography of biologically important RNA molecules has been hampered by technical challenges, including finding heavy-atom derivatives to obtain high-quality experimental phase information. Existing techniques have drawbacks, limiting the rate at which important new structures are solved. To address this, we have developed a reliable means to localize heavy atoms specifically to virtually any RNA. By solving the crystal structures of thirteen variants of the G*U wobble pair cation binding motif, we have identified a version that when inserted into an RNA helix introduces a high-occupancy cation binding site suitable for phasing. This "directed soaking" strategy can be integrated fully into existing RNA crystallography methods, potentially increasing the rate at which important structures are solved and facilitating routine solving of structures using Cu-Kalpha radiation. This method already has been used to solve several crystal structures.

PubMed: 17637337
DOI: 10.1016/j.str.2007.06.003

実験手法

X-RAY DIFFRACTION (2.5 Å)