2PXK

Variant 8 of Ribonucleoprotein Core of the E. Coli Signal Recognition Particle

Summary for 2PXK

• Entry DOI: 10.2210/pdb2pxk/pdb
• Related: 1DUL
• Descriptor: 4.5 S RNA, Signal recognition particle protein, COBALT HEXAMMINE(III) (3 entities in total)
• Functional Keywords: gu pair, hexamine, rna phasing, rna, cation binding, signaling protein-rna complex, signaling protein/rna
• Biological source: Escherichia coli; More
• Cellular location: Cytoplasm: P0AGD7
• Total number of polymer chains: 2
• Total formula weight: 29349.97

Authors

Keel, A.Y.,Rambo, R.P.,Batey, R.T.,Kieft, J.S. (deposition date: 2007-05-14, release date: 2007-08-07, Last modification date: 2024-10-30)

Primary citation

Keel, A.Y.,Rambo, R.P.,Batey, R.T.,Kieft, J.S.
A General Strategy to Solve the Phase Problem in RNA Crystallography.
Structure, 15:761-772, 2007

PubMed Abstract: X-ray crystallography of biologically important RNA molecules has been hampered by technical challenges, including finding heavy-atom derivatives to obtain high-quality experimental phase information. Existing techniques have drawbacks, limiting the rate at which important new structures are solved. To address this, we have developed a reliable means to localize heavy atoms specifically to virtually any RNA. By solving the crystal structures of thirteen variants of the G*U wobble pair cation binding motif, we have identified a version that when inserted into an RNA helix introduces a high-occupancy cation binding site suitable for phasing. This "directed soaking" strategy can be integrated fully into existing RNA crystallography methods, potentially increasing the rate at which important structures are solved and facilitating routine solving of structures using Cu-Kalpha radiation. This method already has been used to solve several crystal structures.

PubMed: 17637337
DOI: 10.1016/j.str.2007.06.003

Experimental method

X-RAY DIFFRACTION (2.5 Å)